Illumina HiSeq 2500 sequencing; Identification of microRNAs in ichthy... - SRA

ERX9131410: Illumina HiSeq 2500 sequencing; Identification of microRNAs in ichthyosporean species
1 ILLUMINA (Illumina HiSeq 2500) run: 191.4M spots, 9G bases, 2.8Gb downloads

Submitted by: University of Oslo

Study: Identification of microRNAs in ichthyosporean species

PRJEB51319 • ERP135937 • All experiments • All runs

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Using Next Generation Sequencing to identify novel microRNA genes in various species of the unicellular eukaryote group of Ichthyosporea.

Sample: Sar degradome

SAMEA14037668 • ERS11640948 • All experiments • All runs

Organism: Sphaeroforma arctica JP610

Library:

Instrument: Illumina HiSeq 2500

Strategy: OTHER

Source: TRANSCRIPTOMIC

Selection: other

Layout: SINGLE

Construction protocol: mRNA was selected using oligo (dt) primers. Then, uncapped (i.e. cleaved or degraded) mRNAs were selected for by ligating a sequencing adapter on the 5' end of mRNAs with a 5'-monophosphate. These ligated mRNAs were then converted to cDNA using random primers and prepared into 50 bp single-end sequencing libraries. The libraries were sequenced on the Illumina HiSeq 2500 platform.

Runs: 1 run, 191.4M spots, 9G bases, 2.8Gb

Run	# of Spots	# of Bases	Size	Published
ERR9581036	191,368,431	9G	2.8Gb	2023-01-03

ID:: 26034565

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